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<p class="MsoNormal" style="background:white"><b><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222">Description<o:p></o:p></span></b></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222">This position will provide molecular biology expertise to the Human Health and Performance Contract (HHPC) at NASA Johnson Space Center
(JSC) in support of NASA human spaceflight programs. As a senior position, the molecular biologist will be responsible for working with the NASA Technical Monitor and Microbiology Laboratory to implement multiple NGS projects using various platforms. These
duties will include implementing routine molecular biology techniques such as nucleic acid purification, PCR, NGS library preparation, and quantification. Additionally, the successful candidate will be expected to operate and maintain Illumina and Oxford Nanopore
Technologies sequencing platforms. While many protocols are already in place, there will be opportunities to support the development of molecular assays for NASA analogs and spaceflight missions. <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222"><o:p> </o:p></span></p>
<p class="MsoNormal" style="background:white"><b><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222">DUTIES AND RESPONSIBILITIES</span></b><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222"><o:p></o:p></span></p>
<ul type="disc">
<li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Perform DNA and RNA extractions from various sample types<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Perform PCRs and assess DNA and RNA quality (Agilent QC and Qubit QC)<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Perform library preparation for Illumina sequencing <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Perform library preparation for nanopore sequencing <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Perform sequencing runs on both Illumina and nanopore platforms <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Prepare new sequencing workflows as required by various projects <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Use and maintain a liquid handling system (epMotion) for Illumina sequencing preparation <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Support the development of molecular assays for NASA analogs and spaceflight <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Write technical reports and method/instrument SOPs<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Demonstrate the ability to resolve project hurdles by applying technical expertise in molecular biology<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Accountable for the accuracy, organization, and interpretation of data<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Conduct laboratory routine instrument maintenance, reagent testing, and consumable tracking to maintain production without interruption<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l0 level1 lfo1;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Deliver high-quality results through independent and collaborative efforts<o:p></o:p></span></li></ul>
<p class="MsoNormal" style="background:white"><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><b><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222">Education/Experience</span></b><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222"> <o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><b><i><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222">Required:</span></i></b><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222"><o:p></o:p></span></p>
<ul type="disc">
<li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l1 level1 lfo2;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">A Ph.D. with 2 years of experience, an M.S. with 5 years of experience, or a B.S. with 10 years of experience in molecular biology, microbiology, biology, biochemistry, cell biology, or a related
field <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l1 level1 lfo2;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Extensive demonstrated expertise with molecular biology techniques such as nucleic acid purification, PCR, NGS library preparation, and quantification <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l1 level1 lfo2;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Demonstrated expertise with Illumina sequencing, sample preparation through sequencing <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l1 level1 lfo2;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Experience troubleshooting Illumina sequencing runs<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l1 level1 lfo2;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Excellent communication and presentation skills<o:p></o:p></span></li></ul>
<p class="MsoNormal" style="background:white"><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222"><o:p> </o:p></span></p>
<p class="MsoNormal" style="background:white"><b><i><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222">Desired</span></i></b><span style="font-size:10.5pt;font-family:"Open Sans",sans-serif;color:#222222">: <o:p></o:p></span></p>
<ul type="disc">
<li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Background in Microbiology<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Experience with microbial metagenomic sequencing <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Experience with nanopore sequencing <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Experience with cell culture<o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Experience with flow cytometry <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Experience using and maintaining a liquid handling device <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Ability to assist with NGS data analysis <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Experience performing qPCR assays <o:p></o:p></span></li><li class="MsoNormal" style="color:#222222;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;mso-list:l2 level1 lfo3;background:white">
<span style="font-size:10.5pt;font-family:"Open Sans",sans-serif">Demonstrated ability to support the writing and publication of scientific manuscripts<o:p></o:p></span></li></ul>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"><a href="https://recruiting.paylocity.com/Recruiting/Jobs/Details/978725">https://recruiting.paylocity.com/Recruiting/Jobs/Details/978725</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"><o:p> </o:p></span></p>
<p class="MsoNormal" style="line-height:105%"><b><span style="font-family:"Century Gothic",sans-serif;color:blue">Aaron B. Regberg PhD (He/Him)<o:p></o:p></span></b></p>
<p class="MsoNormal" style="line-height:105%"><span style="font-size:8.0pt;line-height:105%;font-family:"Century Gothic",sans-serif">Geomicrobiologist, Planetary Protection Lead, Astromaterials Acquisition & Curation Office<o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:105%"><span style="font-size:8.0pt;line-height:105%;font-family:"Century Gothic",sans-serif"><a href="https://ares.jsc.nasa.gov/"><span style="color:blue">Astromaterials Research and Exploration Science (ARES) Division</span></a><o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:105%"><span style="font-size:8.0pt;line-height:105%;font-family:"Century Gothic",sans-serif">NASA Johnson Space Center<o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:105%"><i><span style="font-size:8.0pt;line-height:105%;font-family:"Century Gothic",sans-serif">Mail Code XI2, Building 31, Room 265<o:p></o:p></span></i></p>
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